Strains were maintained on YPD agar plates, containing
10 g L−1 yeast extract, 20 g L−1 peptone, 20 g L−1 glucose and
20 g L−1 agar, and stored at −80 °C in YPD medium containing
10 g L−1 yeast extract, 20 g L−1 peptone and 20 g L−1 glucose
supplemented with 30 % (v/v) glycerol