To reveal the sites of antibody-bound, a solution of PBS containing
0.025% of chromogen diaminobenzidine (DAB,Sigma)
and 0.02 % of hydrogen peroxide was added to the wells.
The reaction was monitored under ligh tmicroscope and stopped by washing the plate with water. Images were obtained with Image-ProPlus 4.5 software(Media Cybernetics,USA)