The fatty acid amides were contained in three fractions: the 70:30 (v/v) hexane:ethyl acetate fraction, 2:1 (v/v) chloroform:isopropanol fraction, and methanol
fraction. These three fatty acid amide-containing fractions were
combined, dried under N2 at 40 C, and then stored at 20 C until
analyzed in more detail. The lipids from 1.0 g of Drosophila media
were extracted as described above to ensure that the Drosophila
were not being exposed to arachidonic acid via the media.
2