The degree of total MHC cross-links (reducible and non-reducible)
was evaluated by comparing the pixel intensity of MHC between samples
without DTT (Fig. 3, right panel). The results showed significantly
higher MHC intensity in the meat emulsions added 100 ppm green tea
extract and significantly lower MHC intensity in meat emulsions
added 1500 ppm green tea extract as compared to the control meat
emulsion. This indicates that meat emulsions added 100 ppm green
tea extract were subjected to less total MHC cross-linking as compared
to the control, and that 1500 ppmgreen tea extract resulted in increased
totalMHCcross-linking. Thus, the degree of totalMHCcross-linkingwas
affected by green tea extract in a dose-dependent manner. Also, it was
found that addition of TG resulted in significantly lower totalMHC levels
as compared to the control, indicating a pronounced polymerization of
MHC as was expected.