Four bionomical features are here described for the seven stingless bees that inhabit a new
meliponine beekeeping area: the Argentinean Chaco region. The most commonly visited plants were of
herbaceous habits, predominantly had flowers of white/creamy to yellowish colour, with small-sized flowers
and were of moderate mass-flowering degree. Lestrimelitta chacoana was the only robber species. The most
commonly found nesting substrate was large living tree trunks, and most nests were found at the base of the
trunks. Sometimes, nesting substrates and floral resources were provided by the same woody plant. Cryptic
small- to medium-sized cerumen tube-like unornamented nest entrances were the most commonly found. Nest
defence included aggressive (Scaptotrigona jujuyensis) and docile behaviour; the latter was classified into timid
and non-timid. A key to identify the Meliponini fauna of this region is provided. The regional pattern of
bionomical features seems to be associated with subtropical climate conditions. The majority of these bee
species are suitable for Meliponiculture in the Chaco region.