ผล : Ergothioneine Content of the Mushroom extract and shrimp Muscles. The amount of ERT in the musroom hot water extract used in the feeding trial was 2.05 t 0.24 els, mg/mL. The ERT content of the control and extract supplement fed diets were 0.26 t 0.21 and 2.24 t 0.42 mg/g, respectively of Accumulation of ERT in shrimp muscle during the feeding for trial was also quantified. The ERT content of the muscle of
หัวข้อ 1.1 For the mushroom PPO inhibition activities of the commercial L-ERT. mushroom hot water extract as well as prepared diets used for the feeding experiment are shown in Figure 1. With increasing concentrations of L-ERT. the mushroom PPO activity remarkably declined. At sa the final concentration of the commercial L-ERT in the assay system, 2726 uM. 74% activity of the control was inhibited after 300 s. Polyphenoloxidase activity was inhibited by 50% at 122 5AM of the L-ERTin the assay system(Figure la). In the case n of the mushroom hot water extract, a final concentration of 0.38 mg ERT mL in the assay system inhibited mushroom P ity at 58% of the control after 300 s(Figure 1b). Inhibition of PPOby the extract was also concentration dependent. Effective r concentration of ER Tin the extract at which the mushroom PPO activity was inhibited by 50% was 0.30 mg of ERT!mL in the assaysystem These results suggest that extracts containing ERT as one of their active compounds potently inhibits mushroom PPO activity. Notably, the original PPO activity was inhibited by the control and treated diet residues by 2 and 29%. respectively(Figure 1c, indicating that the addition of mushroom extract to the diet significantly contributed to its inhibitory activity against days mushroom PPO
หัวข้อ 1.2 bppH Radical Scavenging Meeizivy. DPPH radical scaven- nted ging activity of the mushroom hot water cxtract and prepared Was diets were determined(Figure 2). The DPPH radical scavenging activity of the extract was dose dependent. The effectivc concen- Dom tration of ERT in the extract at which DPPH radical was Donn ding scavenged by 50% was 0.07 mg/mL in the assay system. The DPPH radical scavenging activities of the control and extract supplement fed diet residues were 61 and 76%, respectively, At suggesting that the addition of the extract to the diet significantly SSay increased its radical scavenging activity ited Montalin and Hermoeyteeomrs. The effect of diet supple- at mentation with mushroom extract on the s of Kuruma shrimp is shown in Figure 3. At the end of the seven-day feeding 0.38 trial, no significant differences were found between the control PPO tion and supplement fed groups(shrimp fed the diet containing the tive mushroom hot water extract in terms of mortality and hemocyte count of the shrimp PPO Hemolympha Polyphenoloridese-rfetivities. For the enzy- the matic oxidation of catechol, absorbance at 530 nm increased up ERT to 0.14 for the control group, while it was up to 0.08 for the 00m supplement fed group after the 300 s reaction period, suggesting d by that 39% of the activity of the control was inhibitcd. Absorbance vely t to at the end of the reaction period was significantly(p< 0.05 lower in the supplement fed group than in the control group. The same trend was noted when L-DOPA was used as a substrate for the PPO activity in the hemolymph of shrimp from the control and supplement fed groups. These results show that feeding the mushroom extract significantly(p 0.05) reduced the total PPO activity of the control by 36% (Figure 4)