Seasonality of different metabolites according to time of harvest in Calendula officinalis. Means of the response factors with different letters are significantly different. (Tukey, p < 0.05).To identify which metabolites were altered, we performed ANOVA followed by a pos-hoc Tukey's test. Two of the seven compounds showed no statistically significant variations (p < 0.05): rutin and isorhamnetin-3-O-neohesperidoside. The remaining 5 metabolites were significantly increased or decreased in at least one period in response to the test factors (Table 5).