Materials and methods
Healthy and witches’ broom diseased lime (Citrus aurantifolia
Christm) plants were collected from key lime
gardens of Jiroft in the south of Iran.
Single node shoots were excised from mother plans
and surface-sterilized by soaking in a solution containing
6% bleach and 1% tween 20 for 20 min. The explants
were rinsed tree times in sterile distilled water