In all strengths of BAP all explants develop callus. Maximum explants
response was observed in 16 and 17 day after inoculation. The callus initiation
response decreased on either side of this figure either by increasing or
decreasing the time factor. Minimum days recorded were 13 days where leaf
blade and petiole whereas maximum days consumed for callus initiation were 18
days by explant node. Callus colour was governed by the explant used. In leaf,
petiole and blade, it was green (11 out of 12 cases). In node and internode it was
yellow green (2) to light green (6) and green (4). Texture of the callus was again
explant oriented. Under all BAP concentrations, explants petiole and leaf, mostly
produced granular callus whereas internodes and nodes produced compact
callus.
In all strengths of Kinetin all explants developed callus though varying in
amount. Maximum explants response was observed in 18 day after inoculation.
Minimum days recorded were 15 days whereas maximum days consumed for
callus initiation were 20. Leaf developed callus in minimum (15 days) time while
node developed callus in maximum time (20 days). Callus colour was again
found to be governed by the explant used. In leaf, petiole and blade, it was green
in all explants. In node and internode it was yellow green (3 readings) to light
green (8 readings) and green (1 readings). Texture of the callus was again
explant oriented. Under all Kinetin concentrations applied explants, petiole and
leaf produced 100% granular callus whereas internodes and nodes produced
100% compact callus.