Peroxide value analysis of emulsions
Peroxide value s of the lipid (soybean oil) portion of each emulsion were determined using the ferric thiocyanate method, developed by the International Dairy Federation (IDF) and described in Shantha and Decker (1994). A calibration curve was constructed using 1.0 to 35.0 ug ferric chloride. Emulsions (0.1 mL) were analyzed by dissolving into 9.8 mL chloroform:merhanol (7:3v/v) when sample absorbance exceeded the limits of the calibration curve, emulsions were diluted as appropriate to remain in the calibration range. Each treatment replicate (n=3) was analyzed in duplicate.