Three day before the experiment, the strains stored at 70 C in tryptic soy broth (TSB, Difco) containing 25% glycerol and 0.6% yeast extract (YE, Difco) were transferred on BairdeParker Agar (BPA, Difco) plates and incubated at 37 C for 24 h. A single colony was then used for inoculating into10 mL of BHI broth followed by incubation over-night at 37