Introduction
Alcoholism is one of the most frequent addictions and is of great interest to forensic and clinical medicine. Consequently, the research in forensic toxicology has focused on the identification of biochemical markers of ethanol consumption to diagnose chronic heavy alcohol use, dependence or abstinence and to analyze the alcohol rate in cases, where alcohol abuse can be dangerous (e.g. driving, pregnancy, work place and child custody).Alcohol itself cannot be used as an analyte for this purpose because of its high volatility. However, there are several suitable minor metabolites of ethanol from which fatty acid ethyl esters (FAEE) and ethyl glucuronide (EtG) had been thoroughly investigated.The determination of FAEE and EtG allows a discrimination between excessive repeated alcohol consumption (above 60 g