Whole-cell amino acids and sugars of the isolate
were prepared according to Lechevalier and Lechevalier
(1970) and analyzed by thin layer chromatography
as described by Staneck and Roberts (1974),
using appropriate controls. Similarly, isoprenoid quinones
were extracted, purified and examined after
Collins et al. (1977) and extracted polar lipids
analyzed using the procedure described by Minnikin
Antonie van Leeuwenhoek
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et al. (1984), as modified by Kroppenstedt and
Goodfellow (2006). Cellular fatty acids were
extracted, methylated and separated by gas chromatography
using an Agilent Technologies 6890 N
instrument, fitted with an autosampler and a 6,783
injector, according to the standard protocol of the
Sherlock Microbial identification (MIDI) system
(Sasser 1990; Ka¨mpfer and Kroppenstedt 1996); the
fatty acid methyl ester peaks were quantified using
TSBA 5.0 software. The DNA G?C content of the
isolate was determined following the procedure of
Gonzalez and Saiz-Jimenez (2005).