Elemental and compositional analysis of microalgae A Perkin-Elmer 2400 Series CHNS/o elemental analyzer (Waltham, Massachusetts) was used to analyze the elemental compositions of microalgal biomass. The protein content of microalgae was determined by Dumas method [12]. The moisture content of the biomass was determined by the method of LAP #001, which is the Laboratory Analytical Procedures (LAPs) developed the National Renewable Energy Laboratory [13,14). The ash content of the biomass was determined by the method of LAP 005 [15l