Capture EIA
A capture EIA
Antibody specific for IgG or IgM is attached to a solid-phase surface (plastic bead or microtiter well)
Test specimen containing IgG or IgM is added
Specific antigen is added
An enzyme-labeled antibody specific to the test antigen is added (conjugate)
Chromogenic substrate is added, which in the presence of the enzyme, changes color. The amount of color that develops is proportional to the amount of antigen-specific IgG or IgM in the test specimen.