2. Material and methods
2.1. Strains
The microorganism used at this study was Zymomonas
mobilis NRRL 806. The cells were inoculated in ZM medium:
sucrose (20 g L-1), yeast extract, (10 g L-1) and peptone, (10 g L-1);
the growth was performed at shaker (12.6 rad s-1) for 21 h at
30 C, and stored for no more than 3 weeks in refrigerator
(4 C).
The yeast used at the reactor tests was a strain of
Saccharomyces cerevisiae from Biotechnological Processes
Laboratory e UFPR, named S. cerevisiae LPB1