FFA content, iodine value, peroxide value and saponification number of the oil samples were determined according to the AOCS methods (AOCS, 1995). Palm oil, sesame oil, soya bean oil and sunflower oil were also tested for comparison.
The rice bran oil was analyzed on Zorbax ODS (250mm×4.5mm) by high performance liquid chromatography using mobile phase acetone-acetonitrile 63.6:36.4 with flow rate 1ml/min (Moreau and Powell, 1999). The color of the rice bran oil was determined by UV–vis spectrophotometry. All the experiments were conducted in duplicate to examine the repeatability and the mean value is presented.