The 10.3 m3 aquaponics system was sited in a 116 m2 hoophouse on the grounds of the Cylburn Arboretum in Baltimore, Maryland, U.S. The system was operated with fish and plants for six months (starting in June 2012) prior to the beginning of the study period to allow the biofilter to ripen and nutrient levels to increase sufficiently to support consistent crop growth.