Besides, plant extracts at 10% were also tested in vitro by poisoned
food technique. The plant extracts were subjected to boiling
temperature of 50°C in water bath to avoid contamination and then
they were incorporated into potato dextrose agar (PDA) media by
transferring 2 ml of each type of plant extract in to a Petri dish
containing 20 ml melted warm PDA medium and they were gently
shaken for thorough mixing of the extract. The PDA plates
containing the plant extracts were inoculated aseptically with A.
porri and S. vesicarium by transferring 8 mm diameter agar disc of
7 days old culture of the pathogen to the centre of the Petri dish.
Three replications were maintained for each treatment. The basal
medium (PDA) without any phytoextract served as control. All the
inoculated Petri dishes were incubated at 25 ± 1°C. The radial
growth of the test fungus in the treated plates was measured in all
treatments when the pathogen growth touched the periphery in the
control Petri dishes. The percent inhibition of fungal growth was
estimated by using the formula given by Vincent (1927).