The experiment was carried out from July 6 to August 10, 2002 at the Mariculture
Research Laboratory, Ocean University of China, Qingdao, P.R. China. The shrimp used
in the experiment were collected from the Fengcheng Shrimp Farm, Qingdao. Prior to the
experiment, all the health-selected shrimp (transparent body, active swimming and
feeding) were transferred into aquaria and underwent a 7-day acclimation period during
which they were fed formulated feed (43.39 F 0.22% crude protein , 9.74 F 0.30% fat ,
9.91 F 0.01% ash, 8.41 F 0.06% moisture) to satiation twice a day (at 0600 and 1800 h).
The size of formulated feed is 1– 1.5 mm in diameter and 4 –5 mm in length.