The absorbance detected for a group that received
saline rather than venom was taken as a control of blood loss,and
the amount of bleeding in each procedure was expressed as units
of absorbance in 540nm. For evaluation of the antiophidic activity,
the venom was preincubated at room temperature with Combretum
leprosum extract (30mg/kg) or arjunolic acid(1–30 mg/kg)30min
before the i.v.injection,and a group of animals received oral pretreatment with arjunolic acid(30mg/kg)30min before venom
injection.