The understanding on stoichiometry can give a clearer picture on the effect of CO2 on succinate yield. van Heerden and Nicol (2013) came out a stoichiometry (eq (2)) which claimed to fit the theoretical yield of succinate [41]. This equation proposed 6/7 mol of carbon dioxide reacts with one mol of glucose, producing 12/7 mol of succinate and 6/7 mol of water. Thus, the maximum carbon recovery of succinate per glucose can be as high as 1.71 mol/mol or equivalent to 1.12 g/g as shown in the equation below. However, Eq (2) ignores the formation of biomass.