After radiation treatment, the cotyledons of seeds were resected. The remaining
embryos were then surface sterilized and placed in sterile water for 12 to 16 h. Luhua 11 seeds
with a radiation dose of 0 Gy were used as the control. Soaked embryos were placed in sterile
culture dishes for embryonic leaflet separation. The separated embryonic leaflets were cultured
on somatic embryo induction medium, which contained MS salts, B5
vitamins, 3% sucrose,
0.8% agar, and 10 mg/L 2,4-dichlorophenoxyacetic (2,4-D). Each treatment (radiation dose)
was represented by approximately 90 explants. The culture conditions were as follows: 25 ±
1°C, 13 h/day illumination, and 3000 lux illumination intensity.