Determination of total anthocyanins content: The total anthocyanins were determined as reported by Mondello et al. (2000). Ten gm of sample was filtered through glass wool, and the pulp washed with 90 ml of a Ethanol: HCl mixture previously prepared mixing 79.7 ml of anhydrous ethyl alcohol with 20.3 ml of HCl (37%). The absorbance has been measured at 535 nm, by spectrophotometer (Jenway 6405 UV/visible, Taiwan), using 1 cm cells. The calibration was done with respect to standard curve of pelargonidin 3-glucose (Pg 3-G). The results were expressed as Pg 3-G equivalent (mg per 100 ml of sample).