2.1.2. ACC deaminase activity
The presence of ACC deaminase activity was determined as
described by Dell’Amico et al. (2005), using Pseudomonas putida
S-313 as a reference strain (Penrose and Glick, 2003). The strains
were first cultured in enriched medium (Modified Nutrient Broth or
mNB contains 3.0 g beef extract, 5.0 g peptone, 5.0 g yeast extract,
0.5 g glucose, and 0.5 g sucrose in 1 L of distilled water) for 16 h and
then transferred to N-free DF medium supplemented with 3.0 mM
(filter sterilized) ACC as sole nitrogen source. After inoculation, the
cultures were grown at 28 ◦C with continuous shaking and optical
density at 600 nm was read for 8 days. Growth indicates the
potential for the microorganism to use ACC as a N-source through
deamination.