Biological pre-treatment of organic matter using T. viride in an
aerobic upstream process prior to anaerobic digestion led to a significant
increase in substrate specific methane and gas yield, with
single-parameter variation studies revealing optimal conditions
with an inoculation density of 2 108 spores 25 g1 substrate, an
initial moisture content of 70%, and an aerobic pre-incubation
extending over ten days in total. The improvement in overall efficiency
was highly reproducible within test replications, and thus
a promising technique to pre-treat (ligno) cellulosic feedstock for
enhanced biogas production.