In previous studies it was shown that cinnamaldehyde release is triggered by the presence of ambient moisture [12]. Low relative humidity conditions enable the retention of cinnamaldehyde in the gliadin matrix, and high or medium relative humidity conditions trigger its release. Therefore, in order to simulate their potential
use as antimicrobial food packaging materials, gliadin films were conditioned during one week at 75% RH and 23 C (common conditions that can be achieved inside a packaged foodstuff of medium aw) to trigger the release of cinnamaldehyde, and to achieve a remaining level after their use in accordance to their application.
After the use simulation step, films were conditioned at 0% RH and 23 C with P2O5 until achieving a constant weight, reporting all data in dry basis.The carbon content of the film samples was measured with an elemental analyzer EA 11