Transmission Electron Microscopy (TEM) of Left Ventricle (LV) Myocardium
LV tissue samples were prepared, sectioned and stained as previously described [18]. Ultrathin sections were cut to a thickness of 85 nm using an ultramicrotome and diamond knife. A JOEL 1400-EX transmission electron microscope (Joel, Tokyo, Japan) was utilized to capture and analyze three fields randomly chosen per mouse.