1. Use required number of Wells and keep the remaining with desiccants in tightly closed sealed bag. Consider three consecutive Wells in first strip as Blank, positive and Negative control serum respectively and the next two Wells for Cut off control serum. Rest of Wells are used for diluted sample. Test steps should be done sequentially.
2. Dilute sample 1:101 with sample diluent. Kit's control sera are ready to use and do not nmeet any dilution.
3. Each control sera (positive, negative and cut off) as well as diluted test sera into appropriate well but nothings into blank well.
4. Seal the plate with cardboard sealer tightly. Mix gently and leave well for 30minutes at 37°C.
5. Remove the well content by flicking plate contents into a waste container. Rinse and flick the microtiter well 5 times.