Brown rice was obtained from LPN, Kajang, Selangor,Malaysia. Rice bran was obtained by milling brown rice in a Grain Testing Mill (Sateka). Milled rice bran was then passed through a 600 mm sieve to achieve appro-priate particle sizes. Defatting was immediately carried out using of Soxhlet apparatus utilizing n-hexane as a solvent. This step is crucial to prevent the development off-favour in the bran as a result of hydrolysis of its oil. The dry defatted rice bran was then kept in a sealed container in a desiccator until further treatments/analysis was performed.