Two grams of powdered leaf tissue was homogenized with a polytron (Brinkman model PT3100) at maximum speed for 30 seconds (twice, 10 seconds apart) with 20 ml of cold acetone containing 10% TCA (trichloroacetic acid) and 0.07% ME (2-mercaptoethanol).
Two grams of powdered leaf tissue was homogenized with a polytron (Brinkman model PT3100) at maximum speed for 30 seconds (twice, 10 seconds apart) with 20 ml of cold acetone containing 10% TCA (trichloroacetic acid) and 0.07% ME (2-mercaptoethanol).