most of these cases, embryos started producing callus instead of
germination. Similarly, various treatments tested to improve
germination frequency, such as desiccation of embryos, long
incubation of embryos in the induction medium (4–5 mo),
changing the medium to SH with different growth regulators
were also attempted. Yet none of the above treatments resulted
in conversion of embryos into plantlets. However, when embryos
were transferred to MS medium containing GA3
(14.43 μM), root pole elongation (Fig. 1H ) occurred within
3–5 d in most of the embryos. Sporadic development of shoots
occurred in a period of 2–3 wk when embryos were transferred
to medium containing 2.22 μM BA+2.68 μM NAA (2%;
Fig. 1I–K), 4.54 μM TDZ+0.53 μM NAA+200 mg/L
glutamine (3%; Fig. 1L), or 44.39 μM BA+0.53 μM
NAA+200 mg/L glutamine (3%; Fig. 1M, N).