2.5. Statistical analysisStatistical analysis was performed using the SPSS version 20.0 soft- ware (IBM Corp., Armonk, USA). Descriptive data were expressed in mean ± standard deviation (SD), median (min-max) or number and frequency, where applicable. The normality distribution of the data was analyzed using the Kolmogorov-Smirnov Test. The paired samples t-test was used to compare the values before and after among the groups with normally distributed data. Analysis of variance (ANOVA) was used to compare significant differences among the groups. A repeated measures ANOVA was used to examine recurrent measurements. A p value of