2.3. Selection of potential strain for production of bioethanol
In order to select the potential strain for bioethanol production,
a primary experiment was done by using two different
strains of yeasts following the method of Caputi et al.
(1968). In this method, an inoculum of 1 ml of the yeast
(23 · 103 CFU ml1) was enriched in Yeast Malt Broth production
medium (Dextrose-5.0 g, Peptone-5.0 g, Yeast extract-
3.0 g and Malt extract-3.0 g in 1000 mL distilled water
added with 50% seawater for marine derived strain and distilled
water for terrestrial strain). The fermentation was carried
out in 500 mL Erlenmeyer flasks using 100 mL of
medium. It was kept for fermentation at 28 C on a shaker
at 120 rpm in triplicate. The level of ethanol in the distilled
culture filtrate was tested in a gas chromatographically after
120 h of incubation.