Isolation of Endophytic Fungi
The most important step for the isolation of endophytic fungi that reside in plant tissues is surface sterilization and the plant parts under investigation should be cut into small pieces to facilitate sterilization and isolation processes. To achieve complete surface sterilization, there are various methods to eliminate most of the epiphytic fungi from the exterior tissues and encourage the growth of the internal mycota, according to the type of tissue as well as its location (Strobel 2003, Strobel & Daisy 2003).