Real-time PCR is technique amplify DNA as detect PCR product in real time. This step is analysis age determine of bloodstain by real time-PCR.
Star with draw blood on fabrics, then store at 37 0C and Variation 0-150 day. Next to extract RNA from bloodstain in fabric as then synthesis cDNA, After analysis by real-time PCR.
Expected GAPDH over expression, but CD93 may be decrease expression. And then calculate ratio of Ct value of HBB and 18S since 0-150 day. And we has standard graph.