The Mr of the genome constitutes 27-40% of the virion by weight (M component), or 42-46% of the virion by weight (B component). The genome is segmented; bipartite, segements are distributed among 2 particle types of linear, positive-sense, single-stranded RNA. Minor species of non-genomic nucleic acid are also found in virions, or not found in virions. The encapsidated nucleic acid is mainly of genomic origin, but virions may also contain satellite RNA. The complete genome is 10100-12820-16900 nucleotides long. The RNA-1 is fully sequenced, complete sequence is 5300-7549-9500 nucleotides long. RNA-2 is sequenced, but only an estimate is available, complete sequence is 3774-5214-7500 nucleotides long. The genome has a base ratio of 21.6-24.42-26.6 % guanine; 21.4-23.98-26 % adenine; 19-22.07-25.6 % cytosine; 27.9-29.54-31 % uracil. Nucleotide sequences at the 3'-terminus are virtually identical (in the non-coding region). The 5'-end of the genome has a on each RNA species genome-linked protein (VPg). The 3'-terminus has conserved nucleotide sequences; in all RNA species. The 3'-terminus has on each RNA species a poly (A) tract on each RNA sequence. The 3'-terminus has no tRNA-like structure. The multipartite genome is divided among different particles, each segment encapsidated separately and the segments are distributed between 2 different types of particles. The largest particles contain each one molecule of RNA-1 (sedimenting component B), or one molecule each of RNA-1 and RNA-2. The medium sized particles contain one molecule of RNA-2, or two molecules of RNA-2 (if the RNA is smaller than 4kb). The smallest particles contain no nucleic acid and are found in the top (sedimenting component T) band after sedimentation.
The Mr of the genome constitutes 27-40% of the virion by weight (M component), or 42-46% of the virion by weight (B component). The genome is segmented; bipartite, segements are distributed among 2 particle types of linear, positive-sense, single-stranded RNA. Minor species of non-genomic nucleic acid are also found in virions, or not found in virions. The encapsidated nucleic acid is mainly of genomic origin, but virions may also contain satellite RNA. The complete genome is 10100-12820-16900 nucleotides long. The RNA-1 is fully sequenced, complete sequence is 5300-7549-9500 nucleotides long. RNA-2 is sequenced, but only an estimate is available, complete sequence is 3774-5214-7500 nucleotides long. The genome has a base ratio of 21.6-24.42-26.6 % guanine; 21.4-23.98-26 % adenine; 19-22.07-25.6 % cytosine; 27.9-29.54-31 % uracil. Nucleotide sequences at the 3'-terminus are virtually identical (in the non-coding region). The 5'-end of the genome has a on each RNA species genome-linked protein (VPg). The 3'-terminus has conserved nucleotide sequences; in all RNA species. The 3'-terminus has on each RNA species a poly (A) tract on each RNA sequence. The 3'-terminus has no tRNA-like structure. The multipartite genome is divided among different particles, each segment encapsidated separately and the segments are distributed between 2 different types of particles. The largest particles contain each one molecule of RNA-1 (sedimenting component B), or one molecule each of RNA-1 and RNA-2. The medium sized particles contain one molecule of RNA-2, or two molecules of RNA-2 (if the RNA is smaller than 4kb). The smallest particles contain no nucleic acid and are found in the top (sedimenting component T) band after sedimentation.
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