High-performance liquid chromatography, or HPLC, is a long name for a powerful technique based on the simple fact that individual compounds behave differently in water.
HPLC separates and purifies compounds according to their polarity, or their tendency to like or dislike water. To put polarity into context, consider that oil is an apolar liquid that doesn’t mix with water. Ethanol, on the other hand, is polar and as many of you know, mixes very well with water (Vodka and coke anyone??).
I have tried to simplify the whole process in Figure 1 below, but first let’s look at the main components involved in HPLC. Apologies in advance for some unavoidable jargon!
High-performance liquid chromatography, or HPLC, is a long name for a powerful technique based on the simple fact that individual compounds behave differently in water.HPLC separates and purifies compounds according to their polarity, or their tendency to like or dislike water. To put polarity into context, consider that oil is an apolar liquid that doesn’t mix with water. Ethanol, on the other hand, is polar and as many of you know, mixes very well with water (Vodka and coke anyone??).I have tried to simplify the whole process in Figure 1 below, but first let’s look at the main components involved in HPLC. Apologies in advance for some unavoidable jargon!
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