Two confirmed M. tuberculosis-infected black rhinoceros and one exposed suspect were evaluated for antibody
responses using a lateral-flow rapid test (ElephantTB STAT-PAK) and multi-antigen print immunoassay
(MAPIA).
All three animals were seropositive by both tests. MAPIA detected antibodies to ESAT-6, CFP10, and
MPB83 antigens.
When the rhinos were treated with antitubercular therapeutics, their antibody responses gradually
declined.
One rhinoceros died approximately 9 mo after initiation of treatment and showed an increase in antibody
titer shortly before death.
The other two rhinoceros, which were treated for 1 and 2 yr, respectively, had no clinical
signs or positive culture for M. tuberculosis at the time of necropsy performed 2 or 6 yr later for unrelated reasons.
The antibody levels in these rhinos continued to be significantly decreased.
The findings suggest that the Elephant TB STAT-PAK and MAPIA may be useful tools to detect M. tuberculosis infection and monitor treatment in black rhinoceros.
Two confirmed M. tuberculosis-infected black rhinoceros and one exposed suspect were evaluated for antibodyresponses using a lateral-flow rapid test (ElephantTB STAT-PAK) and multi-antigen print immunoassay(MAPIA). All three animals were seropositive by both tests. MAPIA detected antibodies to ESAT-6, CFP10, andMPB83 antigens. When the rhinos were treated with antitubercular therapeutics, their antibody responses graduallydeclined. One rhinoceros died approximately 9 mo after initiation of treatment and showed an increase in antibodytiter shortly before death. The other two rhinoceros, which were treated for 1 and 2 yr, respectively, had no clinicalsigns or positive culture for M. tuberculosis at the time of necropsy performed 2 or 6 yr later for unrelated reasons.The antibody levels in these rhinos continued to be significantly decreased. The findings suggest that the Elephant TB STAT-PAK and MAPIA may be useful tools to detect M. tuberculosis infection and monitor treatment in black rhinoceros.
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