Chitosan is known as a cheap, eco-friendly and efficient biosorbent
for pollutant mixtures. In the treatment of textile effluents
treatment, it has been widely applied for dye removal and/or
decolorization [1]. The amino (–NH2) and hydroxyl (–OH) groups
of chitosan serve as the active sites for biosorption. The chemical
modification of this water-insoluble material can be done easily
because of the presence of these groups [2–4]. Chitosan is soluble
in an acetic acid solution, and its hydrogel beads are precipitated
and formed in NaOH solution. The elemental structure of chitosan
has no difference after this acidic modification. The chitosan beads
(CBs) have a higher adsorption capacity than the raw chitosan
flakes. Laccase has been of interest in recent years for synthesizing various
dyes and decolorizing industrial textile effluents [5]. It catalyzes
the oxidation of substrates coupled with the reduction of
molecular oxygen from water. Laccase was effectively and popularly
used for dye removal [6,7]. Under alkaline conditions, the
hydroxide ion causes enzyme inhibition [8]. A decrease or loss in