4. Results and discussion
4.1. Degree of deacetylation
Chitosan (0.2 gm) was dissolved in 20 ml 0.1 M hydrochloric acid and 25 ml of deionized water. After 30 minute of
continuous stirring additional 25 ml of deionized water was added and stirring was continued for next 30 minutes.
When chitosan was completely dissolved solution was titrated with 0.1 M sodium hydroxide solution. Degree of
deacetylation (DA) was calculated using formula.