Each isolated strain of Bacillus spp. was incubated in 10 mL BHI medium for bacteriological culture at 30 °C for 24 h. Subsequently, 100 μL of the isolates was seeded on Tryptone Soy Agar (TSA) plates and incubated at 28 °C for 24 h. The pathogens were diluted 100-fold, at a ratio of 1:10 in “Brain Heart Infusion ” Broth (HiMedia, Mumbai); 100 μL of the pathogenic bacteria was seeded in TSA culture medium and incubated for 2 h at 30 °C.