Free radical scavenging activity of extracts was
assessed using the procedure reported earlier with a slight modification (Gulluce et al., 2007). Briefly, 50 μl of sample with five different concentrations (0.1, 0.3, 0.5, 0.7 and 1% v/v) were mixed with 150 μl of a 0.2 mM methanolic solution of DPPH. The mixtures were incubated in the dark condition at room temperature for 30 min. Then, the absorbance at 517 nm was recorded. The DPPH radical scavenging activity was calculated according to the following equation and IC50 was expressed as the concentration of 50% of DPPH radical scavenging activity.