ABA was eluted with 1.5 ml of 80% methanol and vacuumed to dryness again, redissolved in 40l of 20% acetonitrile and injected 5l into a
Waters Acquity SQD UPLC-MS system (Waters, Milford, USA),
a BEHC18 (100mm×2.1mm, 1.7m) column was used in
the UPLC,the mobile phase was 20% acetonitrile. Flow rate
was 0.25ml/min and the column temperature was 30 ◦C.
ABA was eluted with 1.5 ml of 80% methanol and vacuumed to dryness again, redissolved in 40l of 20% acetonitrile and injected 5l into aWaters Acquity SQD UPLC-MS system (Waters, Milford, USA),a BEHC18 (100mm×2.1mm, 1.7m) column was used inthe UPLC,the mobile phase was 20% acetonitrile. Flow ratewas 0.25ml/min and the column temperature was 30 ◦C.
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