. Disinfection of TMV on scissors
Scissors contaminated with TMV (by dipping in TMV suspension for 1 min) were dipped in 100-fold dilution CZ, nutrient broth medium or water for 30 s and were used to cut the first three leaves of N. tabacum cv. NC89 plants (cut off half of every leaf). Scissors dipped in TMV suspension only were used as controls. Each treatment consisted of twenty-five plants and was repeated three times. Disease severity was recorded 15 days after inoculation.