Fig. 1. Anti-M. hyopneumoniae IgG, IgM and IgA responses in serum of pigs
experimentally challenged with M. hyopneumoniae. (A) IgG values; (B)
IgM values; (C) IgA values. The M. hyopneumoniae Tween 20 antigen
(strain 232) was used in this assay. Mouse anti-pig IgG and goat antiswine
IgM and IgA conjugates were used. A cut-off OD value of 0.25 has
been previously validated for serum IgG and was used also for IgM and IgA
values. Thus, OD values above 0.25 are considered positive for all antibody
isotypes. Data represents the mean of duplicate wells for each animal.