The nutrient medium may comprise carbon sources (e.g.
glucose, fructose, maltose, lactose, citric acid, fumaric acid),
nitrogen sources (e.g. corn steep liquor, yeast extract, pep
tone, polypeptone, casein hydrolysate, amino acid) and inorganic salts (e.g. sodium chloride, potassium phosphate, magnesium sulfate). Thus, the nutrient medium may be a conventional one employed for cultivation of bacteria. The addition
, of an amino acid such as asparagine or glutamine is particularly preferred for increasing the yield of asparaginase.