Composition of the production medium used was Tyrosine agar
All the experiments were
carried out in 500 mL Erlenmeyer flasks containing 100 mL of Tyrosine
agar medium. Sterile medium was inoculated with 5% of
inoculum (3.1–4.7
×
104 CFU/mL), incubated at 28 ◦C and cultivated
under agitation at 180 rpm for 7 days. Growth and melanin production
were determined from the samples collected at 12 h intervals
for which samples (5 mL) were taken from each of three replicate
tubes. The cells were harvested by centrifugation at 12,000
×
g for
15 min, at 4 ◦C.