With a run time of approximately 15 h, 10 μm high lateral resolution AP-MALDI images of Glycyrrhiza glabra rhizome were acquired, resolving cellular level localization of flavonoids in the rhizome sections (B. Li, Bhandari, Janfelt, Römpp, & Spengler, 2014). A number of free flavonoids were detected only in the cork layer of the rhizome, but abundant flavonoid glycosides, such as liquiritin and its isomers (neoliquiritin/isoliquiritin/neoisoliquiritin), were detected in regions of the medulla, xylem, and phloem rays (B. Li et al., 2014). This complementary distribution pattern provides hints for deciphering the biological functions of aglycones and related glycosides. In addition, by using the blotting method, the spatial distributions of major interior bioactive natural products underneath the wax layer of the ginkgo leaf were mapped. Biflavones were mainly localized in the stem of the leaf, while quinic acid was detected throughout the leaf blade (Cabral et al., 2013). The spatial information obtained by the various MSI techniques will undoubtedly contribute to our knowledge of flavonoid-related gene expression patterns, and will lead to a better understanding of their biological significance in foods.