For the 24-h and 48-h sampling strategies (Fig. 7 and Fig. 8), all 4 graphs showed a small positive bias. This bias is due to the fact that these sampling strategies only take into account hour measurements started on specific days, while the true EF is based on continuous data over all days of the week. Furthermore, the lower and upper uncertainty bounds coincided for all 4 graphs. This is because, for each of the 1000 iterations, there is only one way to sample the maximum of n 24-h/48-h sampling periods. When the maximum number of n 24-h/48-h sampling periods was sampled, only one estimate of the EF (and associated relative error) was found. In contrast to the previous sampling strategies, the lower and upper uncertainty bounds did not coincide for both graphs ( Fig. 9) when the maximum number of 7-d periods was reached. This is because a 27 or 28-week sampling strategy can start on each working day of the week. Therefore, the estimates between simulated strategies will differ depending on which day of the week the strategy started. If the maximum number of single grab samples would be taken into account, the lower and upper uncertainty bounds would coincide for both graphs (Fig. 10). As for the 7-d sampling strategy, the lower and upper uncertainty bounds for the weekly grab sampling strategy (Fig. 11) did not coincide since the number of possible ways to conduct a weekly grab sampling strategy of 35 (dataset 1) or 34 (dataset 2) consecutive weeks is almost infinite